The Effect of Dexamethasone on Glycosaminoglycans of Human Trobeculor Meshwork in Perfusion Organ Culture
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چکیده
s. Invest Ophthalmol Vis Sci 30(Suppl):224, 1989. Downloaded From: http://iovs.arvojournals.org/pdfaccess.ashx?url=/data/journals/iovs/933380/ on 10/02/2017
منابع مشابه
The effect of dexamethasone on glycosaminoglycans of human trabecular meshwork in perfusion organ culture.
The effect of dexamethasone treatment on glycosaminoglycans (GAG) in the human trabecular meshwork was studied by placing 20 pairs of eyes in perfusion organ culture. One eye received 550 nM dexamethasone in addition to culture medium; the fellow eye received culture medium only. 3H-glucosamine and 35S-sulfate were added to the medium for the final 48 hr of culture. The meshwork was then dissec...
متن کاملmRNA in situ hybridization of TIGR/MYOC in human trabecular meshwork.
PURPOSE To determine the distribution of mRNA expression of the trabecular meshwork-induced glucocorticoid response protein/myocilin (TIGR/MYOC) in human trabecular meshwork. METHODS In situ hybridization using a 1.25-kb probe obtained from reverse transcription-polymerase chain reaction of TIGR/MYOC cDNA was performed to determine the location of cell labeling within the different regions of...
متن کاملHuman trabecular meshwork cell survival is dependent on perfusion rate.
PURPOSE To determine whether suppression of flow may be detrimental to trabecular cell survival and to the morphologic characteristics of the trabecular meshwork. METHODS The anterior segments of normal human eye bank eyes were placed in perfusion organ culture. The effect of various perfusion rates of culture medium, and of the constant flow and constant pressure methods of delivery of cultu...
متن کاملEffects of modifiers of glycosaminoglycan biosynthesis on outflow facility in perfusion culture.
PURPOSE Glycosaminoglycans (GAGs) have been implicated in the regulation of outflow resistance of aqueous humor flow through the trabecular meshwork (TM). Their role was further investigated by assessment of the effects of chlorate, an inhibitor of sulfation, and beta-xyloside, which provides a competitive nucleation point for addition of disaccharide units, in anterior segment perfusion cultur...
متن کاملDexamethasone-induced ocular hypertension in perfusion-cultured human eyes.
PURPOSE Glucocorticoid administration can lead to the development of ocular hypertension and corticosteroid glaucoma in a subset of the population through a decrease in the aqueous humor outflow facility. The purpose of this study was to determine whether glucocorticoid treatment can directly affect the outflow facility of isolated, perfusion-cultured human eyes. METHODS The anterior segments...
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تاریخ انتشار 2005